Biochemical Engineering — Summer 2018

Write down Monod Equation explaining each term in it. How do you determine the constant parameters of Monod equation? Is there any limitation of this equation?

Derive the design equation for a chemostat system (continuous stirred tank fermenter) for biochemical process. Show the schematic and material balance with assumptions. Explain ‘critical dilution rate’ and ‘wash out’ conditions.

Draw the differences between a chemical and Biochemical reaction.

Pasteurization, (ii) Oxygen uptake rate, (iii) respiratory quotient,

Discuss the relative advantages and disadvantages of continuous culture.

What is a fed-batch reactor? Explain with diagram various configurations of fed-batch bioreactor.

Define/explain the flowing term:

Explain with a diagram different phases of microbial growth.

Discuss the air sterilization process for a large scale aerobic fermenter with a schematic diagram. Name a few materials used as air filters.

Explain briefly the range of fermentation process.

Enlist various process and monitoring parameters for control of a fermenter.

Draw a schematic of a fermenter vessel. Label the major components and briefly explain their functions.

What is denaturation of proteins? State three factors responsible for denaturation of protein.

What is meant by ‘Aseptic conditions’? State important considerations for maintenance of aseptic conditions.

Explain briefly the principle of scale-up in bioprocess operation.

Explain the effect of various physico-chemical parameters on Ka. l

State various methods of Ka determination for aerobic system. l Discuss any one in details.

Give two examples of industrially important biochemical reaction and name the microorganisms involved thereon.

Name various unit operations involved in upstream and downstream of bioprocess and cite industrial examples of each of them.1

Present a schematic outlines of various steps involved in an integrated bioprocess. Elaborate any two steps with industrial examples.

Explain what is meant by limiting substrate concentration in the growth.

State various strategies of aeration and agitation in fermenter vessel.

State the importance of rheology in fermentation. Explain rheological behavior of fermentation broth with examples.

Name different types of solid media used for growth.

Discuss briefly various constituents of a liquid media used for growth of yeast and give examples.

What is the purpose of cell disruption? Discuss various methods of cell disruption briefly.