Enzymes and Proteins — Summer 2022

Give 3 examples of plant or animal based industrial enzyme and their uses.

Explain the lock and key hypothesis of enzyme substrate reaction.

Write a note on classification of Enzymes

Explain the concept of induced fit hypothesis of enzyme substrate activity.

Compare competitive inhibition with allosteric inhibition.

Derive the Michealis Menten equation for Enzyme kinetics.

Write a note on chemical methods for immobilizing enzymes.

Give three examples of enzymes which are used in the food industry, along with its application.

Compare encapsulation and entrapment methods for immobilizing enzymes.

Explain Sanger’s method for sequencing DNA, with diagram.

How do we carry out Nterminal sequencing of proteins?

What are chaperones? Does the GROEL and GROES complex in E.coli use ATP for carrying out its function?

Explain the different phenomena that happen at protein protein interfaces.

What is the effectiveness factor of an immobilized enzyme? What is effective diffusivity?

Write a note on immobilization of enzymes through adsorption, its advantages and disadvantages.

How can you use enzymes for analytical application, explain using an example.

Explain primary and secondary structures of proteins.

How can we understand about the primary structure of a protein using Mass spectroscopy?

Explain about the tertiary and quarternary structure of proteins and write in brief, how we can use Xray crystallography and NMR to study the 3D structure of proteins.1

What are domains in proteins, explain modular structure of proteins using domains.

Compare crosslinking of enzymes with entrapment of enzymes in gels as method of immobilization.

Write a note on the evolution of visual pigments.

Explain the significance of Km and Vmax.

Explain the industrial uses of amylase and cellulose enzymes.

Write a note on the evolution of the globin fold.