Explain “lock and key” hypothesis.
[3 marks]What are the six classes of enzymes? Give suitable example.
[4 marks]Describe different types of enzyme specificities with examples.
[7 marks]Explain “induced-fit” theory of enzyme action.
[3 marks]Comment on the allosteric regulation of enzyme action.
[4 marks]Give the derivation of Michelis-Menten Equation.
[7 marks]Comment on the formation and stabilization of secondary structure of proteins.
Write a note on multi-subunit protein.
[3 marks]Explain transition state theory of enzyme catalysis.
[4 marks]Describe different physical methods of enzyme immobilization.
[7 marks]Explain the concept of active site and binding site in enzyme.
[3 marks]Explain collision theory of enzyme catalysis.
[4 marks]How will you obtained crude enzyme extracts from microbes?
[7 marks]Write various limitations of enzyme immobilization.
[3 marks]Comment on the analysis of film and pore diffusion.
[4 marks]Comment on the use of enzymes in textile & leather industries.
[7 marks]Write the advantages of enzyme immobilization.
[3 marks]How will you immobilize invertase enzyme?
[4 marks]How will you isolate and purify enzymes from plant sources?
[7 marks]How pH affects the activity of enzyme?
[3 marks]Comment on the diagnostic use of enzymes.
[4 marks]Comment on the survey of protein structures and functions.
[7 marks]How temperature affects the activity of enzyme?
[3 marks]Comment on the analytical use of enzymes.
[4 marks]Explain the concept of protein folding. Comment on the idea of protein design.
[7 marks]