What do you mean by Gene cloning? Explain the steps involved with clean and labelled diagram.
[6 marks]Explain the process of Transcription in detail with clean and labelled diagram.
[5 marks]Write a note on applications of rDNA technology.
[5 marks]Explain the methods used for isolation of DNA with suitable diagram.
[6 marks]Write a note on restriction enzymes their types.
[5 marks]Discuss on DNA delivery methods.
[5 marks]What is PCR? Explain the cycle and write the applications in detail.
[6 marks]What is HUMULIN? How it is produced using rDNA technology.
[5 marks]Discuss DNA vaccine in detail.
[5 marks]What are monocloanl Antibodies? How they are produced? Write the applications of Monoclonal Antibodies.
[6 marks]Discuss Transgenic plants in detail. Write the applications of Transgenic plants.
[5 marks]what do you mean by Gene therapy? Explain the types with suitable examples.
[5 marks]Write a note on DNA sequencing methods. Explain the Maxam Gilbert methods for DNA sequencing with suitable diagram.
[6 marks]Explain in detail about Transgenic animals. Write their applications.
[5 marks]What do you mean by cDNA? How it is synthesized? Q. 6 (a) What are vectors? Write their characteristics. Explain about PBR322 in detail.
[6 marks]What are blotting techniques? Explain Southern blotting in detail with suitable diagram.
[5 marks]Explain about bacteriophage with labelled diagram.
[5 marks]Explain the applications of Gene therapy in hematopoietic disorders.
[6 marks]What do you mean by RNA? Explain the types with their functions.
[5 marks]Write a note on Anti sense therapy with suitable diagram.
[5 marks]