Molecular Biology and Genetics — Summer 2022

Explain Law of Segregation with a suitable illustration.

Explain the terms allele, cistron, homozyogous

In a mice, dominant allele Cmust be present in order for any pigment to be developed in the coat. The kind of pigment depends on another locus thus B- produces black and bb produces brown. Genotype cc produces no pigment called albinos. Ahomozygous black female is test crossed with albino male. What phenotypic ratio is expected in F1 and F2?

Differentiate between epistasis and dominance. Also explain dominant epistasis with suitable illustration.

Draw diagram of Messelson and Stahl's experiment if DNA replication was conservative. (Assume that it is not semi-conservative). (draw diagrams for 1st, 2nd and 10th generation)

07 The above graph is the absorbance of two DNA solutions (Aand B) of same number of base pairs (same bp length fragments) v/s temperature of the solution. Please find out the approximate melting temperatures of Aand Bw. Which of the DNA fragments, Aor Bhas higher cytosine content, give reason for your answer

Define the terms mutation, mutants, mutagen.

Prokaryotes or Eukaryotes, which type of organisms have a gene structure with less information density compared to another? Why can one type afford to have less information density in its gene compared to another?

Explain post transcriptional modification of mRNA, tRNA and rRNA.

Explain the Hypochromic and Hyperchromic effect of DNA.

How does the repair system discriminate between parent and daughter strand for repair?

If proteins were the genetic material in nature, what would the data from Hershey and Chase’s famous blender experiment look like? Explain with diagrams. OR1

Write a short note on one gene one protein hypothesis.

Write a note on prokaryotic RNA polymerase.

Explain mismatch repair with a neat diagram.

Explain wobble hypothesis in detail.

Explain role of helicase and topoisomerase in prokaryotic replication.

Explain suppression of mutation with example

Explain the formation of initiation complex in prokaryotic translation.

Explain transcription termination in prokaryotes by formation of hairpin loop structure. How does termination happen if a hairpin structure is not formed?

Explain functions of all the enzymes involved in prokaryotic replication.

Write a note on pleiotropy.

From what substrates is RNA made? Does it require primer? Does it require template? Which enzyme plays key role in transcription?

Explain the concept of replisome and primosome.

Differentiate between dominance And epistasis.