Biochemical Engineering — Winter 2018

Discuss any two techniques used to extract intracellular product via Cell Disruption.

Explain different methods for the determination of k a L

Discuss the material of construction used in the design of bioreactors with example

Define Precipitation and Filtration techniques used in downstream processing.

Discuss various developments that took place in history for biochemical engineering and its products.

Explain the methods for the maintenance of Asceptic condition and containment

Explain the Lock and Key model with diagram for enzymatic reactions

List various types of valves used in biochemical process industry

Discuss the role of impellers used for agitation in fermentor in brief.

Discuss in detail the difference between batch culture and Fed-batch culture.

Define Monod growth kinetics and its application

Write a short note on OTR and OUR

Discuss in brief the variants of fermentation vessels

Explain in brief what happens during Lag phase and exponential phases of microbial growth

Explain the importance of pH in fermentation or enzymatic process for product formation

Write short note on antibiotics and its application

Discuss the challenges faced during Scale-Up of fermentation process.

Define crystallization and state its application in biochemical industry

Discuss the methods to enhance aeration in a fermentor for an aerobic process.

Define Enzyme inhibition and role of cofactors

Write a brief note on various sterilization techniques of a fermentation process

Discuss various types of steam traps used in fermentation vessel OR1

Explain the effect of Temperature, Substrate concentration and Enzyme concentration for any biochemical reaction

Discuss the importance of Fluid rheology in design of any fermentation process equipment

Discuss the basic functions of a fermenter for microbial or animal cell culture