Discuss principle of GC. What are the requirements of mobile phase in GC?
[6 marks]What is importance of temperature programming in GC? Discuss applications of GC and give difference between GC and GC-MS.
[5 marks]Discuss various types of derivatization in GC. Write in detail about derivatization reaction that improves stability of molecules.
[5 marks]Enlist various theories of HPLC. Elaborately discuss rate theory.
[6 marks]What is Van Deemter plot? Write applications of Van Deemter plot and differentiate normal phase and reverse phase HPLC.
[5 marks]Draw block diagram of HPLC and discuss about stationary phase in detail.
[5 marks]Enlist detectors used in HPLC. Discuss about PDA detector.
[6 marks]Write a note on LC-MS/MS.
[5 marks]Write principle of ion-exchange chromatography and discuss stationary phase used in ion-exchange chromatography.
[5 marks]Define pre-conditioning. What is importance of pre-conditioning in HPTLC. Discuss sorbents used in HPTLC.
[6 marks]Discuss basic principle and applications of Raman spectroscopy.
[5 marks]Write principle and applications of affinity chromatography.
[5 marks]Discuss Bragg’s law. Explain how X-ray is generated in X-ray spectroscopy.
[6 marks]Enlist units of radioactive decay. How radioactivity is measured?
[5 marks]Write a brief note on isotopes dilution analyses. Q. 6 (a) What are the applications of ELISA? Discuss various types of ELISA.
[6 marks]Elaborately discuss applications of radio nuclides.
[5 marks]What is importance of GLP? Enlist ten basic principles of GLP.
[5 marks]Define patent and discuss different steps involved in patent filling process.
[6 marks]Discuss various validation parameters for analytical method validation as per ICH guideline.
[5 marks]Discuss role of ISO 9001:2000 in quality management system.
[5 marks]