Define and explain the following 1. Bathochromic shift 2. Auxochrome 3. Molar Absorptivity
[6 marks]What do you mean by fluorescence? Describe Jablonski diagramme
[5 marks]Discuss the factors affecting the deviation from Beer’s law
[5 marks]Write a note on sampling techniques used in IR spectroscopy
[6 marks]Enlist detectors used in IR spectroscopy and explain any one in detail
[5 marks]Describe various applications of IR spectroscopy
[5 marks]Define quenching. Explain types of quenching.
[6 marks]Enumerate the detectors used in UV spectroscopy and discuss any one
[5 marks]Write a note on Hollow cathode lamp.
[5 marks]Discuss absorption chromatography in detail
[6 marks]Write a brief note on turbidimetry
[5 marks]Write principle, methodology and applications of ion exchange chromatography
[5 marks]Write principle and applications of Flame photometry
[6 marks]Draw a schematic diagramme of Gas chromatography instrument
[5 marks]Discuss theory and applications of Gel chromatography Q. 6 (a) Differentiate between HPLC and GC
[6 marks]Discuss the stationary phases and mobile phases used in reverse phase HPLC
[5 marks]Write theory, instrumentation and applications of affinity chromatography
[5 marks]Define and explain the following 1. Retention factor 2. Theoretical plates 3. Resolution
[6 marks]Describe types of paper chromatography based on the development
[5 marks]Discuss factors affecting electrophoretic mobility
[5 marks]