Discuss principle and pharmaceutical applications of HPLC.
[6 marks]What is retention mechanism in gas chromatography? Explain different stationary phase and gas is used in gas chromatography.
[5 marks]Explain instrumentation with a schematic diagram and applications of HPTLC
[5 marks]State and explain Beer’s law. What are the reasons for deviation from Beer's law?
[6 marks]Draw a well-labeled diagram of fluorimeter and explain function of each component.
[5 marks]Explain the principle of Atomic Absorption Spectroscopy. Discuss applications of AAS.
[5 marks]Discuss the pharmaceutical applications of UV-Visible spectroscopy.
[6 marks]Explain detectors used in UV-Vis spectrophotometer?
[5 marks]Discuss various Detectors and Pumps used in HPLC.
[5 marks]What do you mean by Molecular vibration in IR. Explain types of stretching and bending vibration in IR spectroscopy.
[6 marks]Explain the principle and applications of Ion Exchange chromatography.
[5 marks]Discuss constructions and working of Michelson interferometer.
[5 marks]What is called quenching? Discuss types of quenching of fluorescence with suitable examples.
[6 marks]Explain various types of interferences in flame photometry. Explain flame and nebulizer burner system in flame photometry.
[5 marks]Write applications of paper chromatography. Write different types of paper chromatography. Q. 6 (a) What is the principle of turbidimetric and Nephelometric method? Discuss the sample cells and applications of Nephelometric method?
[6 marks]What is the principle of electrophoresis? Write types of electrophoresis and applications.
[5 marks]Explain the terms used in chromatography: (i) Resolution (ii) Capacity factor (iii) Retention time (ii)Tailing factor
[5 marks]What is affinity chromatography? What are the steps of affinity chromatography? Write applications of affinity chromatography.
[6 marks]What is the principle of gel chromatography? What is another name for gel chromatography? What are the classification of gels in gel chromatography?
[5 marks]Explain concept of HETP. Discuss Rate theory of chromatography.
[5 marks]