What is spectrometry? Classify spectrometry with examples. Explain energy levels of molecules.
[6 marks]Derive beer’s lambert law of UV visible spectrometry. Give its limitations.
[5 marks]Explain chromophores and auxochromes. Convert 250 nm into frequency (Hz).
[5 marks]Write principle of IR spectroscopy. Give modes of vibration of molecules.
[6 marks]Write a note on factors affecting vibrations with examples.
[5 marks]Write a note on theory and instrumentation of atomic emission spectroscopy.
[5 marks]What is chromatography? Classify chromatography with examples.
[6 marks]Give basic principle, methodology, advantages, and disadvantages of TLC.
[5 marks]Write a note on gel chromatography.
[5 marks]Write basic principle involved in HPLC. Draw diagram of HPLC instrument.
[6 marks]Write a note on nepheloturbidometry.
[5 marks]Write a note on detectors used in Gas chromatography.
[5 marks]Write principle, merits, demerits, and applications of ion exchange chromatography.
[6 marks]Write a note on affinity chromatography.
[5 marks]Explain: Rf, Rt, Guard column, Reversed Phase Chromatography, Isocratic mobile phase. Q. 6 (a) Explain effect of solvent and pH on λmax with examples.
[6 marks]Give theory of fluorescence. Write factors affecting fluorescence.
[5 marks]Draw FTIR instrument. Explain detectors used in IR spectrometer.
[5 marks]Write a note on Capillary electrophoresis.
[6 marks]Write a note on monochromators used in UV visible spectrophotometer.
[5 marks]Give IR stretching peaks (cm-1) of C=O, N-H, C-H, C-O, C=C.
[5 marks]