What is molten globule?
[3 marks]Discuss in brief the forces that stabilise protein structure.
[4 marks]Explain allosteric regulation of enzyme action with example.
[7 marks]Discuss secondary structural elements of protein. Define enzyme, cofactor, apoenzyme and holoenzyme and describe different
[4 marks]What are the folding funnels?
[3 marks]7 classes of enzymes.
[ marks]Describe purification of crude enzyme extracts from microbial sources.
[7 marks]Enlist important enzymes in food industry and discuss the importance of any
[ marks]Explain principle of affinity chromatography.
[4 marks]Write the Benefits and limitation of enzyme immobilisation.
[7 marks]Explain Ramachandran map.
[3 marks]Write the factors that affect globin folding patterns.
[4 marks]Enlist various methods of purification of enzymes. Discuss any one in detail
[7 marks]3 one.
[ marks]7 deactivation kinetics.
[ marks]Discuss any protein misfiling disease. Explain the principle and procedure of affinity chromatography for protein
[4 marks]7 purification.
[ marks]Explain multi-subunit proteins
[3 marks]Explain protein-DNA interactions with example. How pH and temperature affects enzyme activity? Comment on the
[4 marks]Explain the principle of thin layer chromatography.
[3 marks]7 significance of it.
[ marks]Explain general properties of protein protein interfaces.
[4 marks]Discuss in detail GroEL-GroES mechanism for protein folding.
[7 marks]What is proteostasis?
[3 marks]Explain thermodynamics of protein folding. Give the derivation of Michelis-Menten equation and comment on the
[4 marks]What is levinthal paradox?
[3 marks]