Explain the role of entropy in catalysis of enzyme.
[3 marks]Explain about specificity of enzymes.
[4 marks]Explain the method of classification of enzyme.
[7 marks]Discuss the significance of Vmax.
[3 marks]Explain features and working of active site.
[4 marks]Compare different plots and modifications used to overcome limitations of Michealis-Menten plot with equation.
[7 marks]What do you mean by enthalpy, entropy and free energy.
[3 marks]Write Michaelis-Menten equation & Write assumptions for derivation of Michaelis-Menten equation.
[4 marks]Explain the concept of standard free energy.
[7 marks]Write a note on Sangers method for sequencing genes.
[3 marks]What are various features of protein protein interface?
[4 marks]Discuss in detail about the factors that affect the rate of chemical reaction.
[7 marks]Which method can give the highest resolution of protein structure? Write a brief note about the same.
[3 marks]Compare NMR and X-Ray crystallography as protein structure tools.
[4 marks]Write a note on CD-Spectroscopy, Maldi-Tof and at least one method for understanding protein primary structure.1
[7 marks]Write note with examples on enzymes from plant source , animal source and microbial source.
[3 marks]Write a note about commercial applications of enzymes in at least two different industries.
[4 marks]Write a note on allosteric activation and inhibition as a means for control of protein activity.
[7 marks]Define enzyme activity, effectiveness factor and domains.
[3 marks]What is Anfinsen’s hypothesis? How it was demonstrated?
[4 marks]Write a note on enzyme immobilization.
[7 marks]Effect of denaturants on protein structure.
[3 marks]How folding funnels describe folding phenomenon?
[4 marks]Explain GroEL GroES system and its function.
[7 marks]