What are the first digits according to Enzyme classification system for all main classes of enzymes?
[3 marks]Which class of enzyme catalyses conversation of Lglutamate to Lglutamine?
[4 marks]Explain the advantages of covalent methods for enzyme immobilization over physical method of immobilization and Explain any two platforms of next generation sequencing
[7 marks]What is turnover number? It is determine by which expression?
[3 marks]Elaborate on the effectiveness factor of an immobilized enzyme.
[4 marks]Derive Michaelis-Menten equation with Briggs-Haldane modification.
[7 marks]Write a detailed note about significance of Michaelis- Menten equation
[7 marks]Draw the principle of Edman degradation for peptide sequencing
[3 marks]Explain encapsulation as a means for immobilization of proteins
[4 marks]Derive the equation of Haldane relationship for reversible reactions.
[7 marks]Draw the principle of Sangers sequencing of DNA
[3 marks]Explain the diagnostic application of enzymes
[4 marks]Explain control of protein activity using an inhibitor
[7 marks]Which enzymes are derived from plant sources, which industries use them? What are the EC numbers of these enzymes
[3 marks]Which type of enzyme immobilization Is used for biodiesel formation, why?
[4 marks]When you want to study the percentage of alpha helices, beta sheets and random coils, which technique will you use? Explain the principle for the same.
[7 marks]Give commercial application of enzymes in food and pharmaceutical industries.
[3 marks]Explain control of protein activity through allosteric interaction.
[4 marks]Justify the statement “ selection has tuned vertebrate opsins so that the adsorption maximum matches the light environment1
[7 marks]How can we use ammonium sulphate for purification of proteins?
[3 marks]Explain the use of X-ray free electron laser for determination of dynamic protein structure determination.
[4 marks]What are different ways by which proteins evolve new functions
[7 marks]Name at least 3 enzymes of microbial origin, and give their industrial use.
[3 marks]Give brief summary of comparative analysis of different globins that depict sequence structure relation and evolution of full length globins.
[4 marks]To what constraints are pathways of protein evolution subject
[7 marks]