Biochemical Engineering-I — Summer 2018

Define the terms: thixotropic and rheopectic fluids

Explain the terms: open system and closed system

Derive Mechalis-Menten equation.

Explain the law of conservation of mass.

Distinguish between biochemical engineer and bioprocess engineer.

Liquid from a brewery fermenter can be considered to contain 10% ethanol and 90% water. 50.000kgh-1 of this fermentation product are pumped to a distillation column on the factory site. Under current operating conditions a distillate of of 45% ethanol and 55% water is produced from the top of the column at a rate one-tenth that of the feed.

What is the composition of the waste “bottoms” from the still?

What is the rate of alcohol loss in the bottoms?

Explain how biologist and an engineer differ in their approach?

Define: time dependent viscosity, elasticity.

What four types of products do biotechnologies produce?

Discuss the non-newtonian fluids with required equations.

Write a note on effects of shear on viscosity for all fluids.

Draw the sketch of bioreactor with necessities.

Discuss the fundamentals of gas-liquid mass transfer.

What is analogy between mass and heat transfer?

Briefly explain : enzyme deactivation

Write a note on: diffusion theory

Elaborate on product formation kinetics

Explain the terms: yield factor, respiratory quotient

Narrate the cell growth kinetics with suitable equations.

What is function of sparger, baffle and pinch valve in a bioreactor?

What criteria should be considered for bioreactor construction?

Derive the expression for batch culture time as a function of product concentration

What is difference between chemostat and turbidostat operation?

What do you mean by probability of contamination? Why it is necessary to consider it in sterilization process?

What is difference between asepsis and sterilization? How can aseptic operation be achieved in a reactor?1