Biochemical Engineering-I — Summer 2019

What is role of bioprocess engineer in an industry?

Explain difference between steady state and equillibrium.

Acontinuous process is set up for treatment of wastewater. Each day, 105 kg cellulose and 103 kg bacteria enter in the feed stream, while 104 kg cellulose and 1.5 x 104 kg bacteria leave in the effluent. The rate of cellulose digestion by the bacteria is 7 x104 kg d-1. The rate of bacterial growth is 2 x 104 kg d-l; the rate of cell death by lysis is 5 x 102 kg d-1. Write balances for cellulose and bacteria in the system.

Explain the terms intensive and extensive properties.

Explain the term Heat of combustion.

Corn-steep liquor contains 2.5 % invert sugars and 50% water; the rest can be considered solids. Beet molasses containing 50% sucrose, 1% invert sugars, 18% water and the remainder solids is mixed with corn- steep liquor in a mixing tank. Water is added to produce adiluted sugar mixture containing 2% (w/w) invert sugars. 125 kg corn-steep liquor and 45 kg molasses are fed into the tank.

How much water is required?

What is the concentration of sucrose in the final mixture?

Water at 25oC enters an open heating tank at a rate of 10 kg h-1. Liquid water leaves the tank at 88oC at a rate of 9 kg h-1; 1 kg h-1 water vapour is lost from the system through evaporation. At steady state, what is the rate of heat input to the system?

What is Reynold’s number? What is its significance?

What is difference between radial flow impeller and axial flow impeller?

What is viscosity? Explain working of Coaxial-cylinder Rotary Viscometer with diagram.

What is role of diffusion in bioprocessing?

Enlist different methods for determination of k a. Explain any one in L detail.

Explain the steps for transfer of gas from bubble to a cell with a neat diagram.1

What do you mean by total rate, volumetric rate and specific rate?

Derive the equation for kinetics of cell death.

Glucose isomerase is used extensively in the USA for production of high-fructose syrup. The reaction is: glucose ↔ fructose. ∆Ho for this reaction is 5.73 kJ gmol-l; ∆So is 0.0176 kJ gmol-l K- rxn rxn 1.

Calculate the equilibrium constants at 50oCand 75oC

Acompany aims to develop a sweeter mixture of sugars, i.e. one with a higher concentration of fructose. Consideringequilibrium only, would it be more desirable to operate the reaction at 50oC or 75oC?

What do you mean by theoretical and observed yields?

Compare the equations of Lineweaver-Burk Plot, Eadie-Hofstee Plot and Langmuir Plot.

What is difference between chemostat and turbidostat?

Explain kinetics of Substrate Uptake in the Absence of Product Formation.

Explain in detail typical batch growth curve and also relate it to specific growth rate.

Give applications of trickle bed, packed bed and fluidized bed reactors?

What considerations must be given for fermenter designing for inoculation and sampling control?

Explain the process of batch heat sterilization of liquids in detail.