Biochemical Engineering-I — Winter 2018

Define the terms: Respiratory quotient, Yield, Growth rate limiting reaction.

Discuss the sterilization of air for the fermentation operation.

Afermenter containing microbial culture at 30 0C is used for production of Gmiivcreo abnia al cicnoseucntti coifd eea wchit hw iittsh 2 s0u-iltiatrbel ec aepxapcliatnya. tKionas i.s to be determined. Air flow L is shut off for few minutes and DO level drops the air supply is then reconnected. When steady state is established, the DO tension is 77% air saturation. The following results are obtained. T T12 Time 5 Oxyge 50 66 n tension Where, C = final steady DO concentration and C = DO AL AL Concentration. (a) Estimate Ka. (b) An error is made determining L steady state oxygen level, which instead of 77% is taken as 69%, what is the percentage error in K L a resulting from this error in C AL.

How do the analogy exists between mass and heat transfer?

Discuss the basic steps for designing of a bioreactor.

The fungus Aureobasidium pullulans is used to produce extracellular polysaccharides by fermentation of sucrose. After 120h fermentation, the following measurements of shear stress and shear rate were made with a rotating cylinder viscometer. Shear Stress dyn Shear Rate cm-2 s-1 44.1 10.2 235.3 170 357.1 340 457.1 510 636.8 1020 [1] Plot the rheogram for this fluid. [2] Determine the appropriate Non Newtonian parameters. [3] find the apparent viscosity at the shear rates of ; 15 s-1 and 200 s-1.

Abacterium converts ethanol to acetic acid under aerobic conditions. A continuous fermentation process for vinegar production is proposed using non- viable bacterial cells immobilized on the surface of gelatin beads. The production target is 2 kg h-1acetic acid; however the maximum acetic acid1 concentration tolerated by the cells is 12%. Air is pumped into the fermenter at a rate of 200 mmol h-1.

What minimum amount of ethanol is required?

What minimum amount of water must be used to dilute the ethanol to avoid acid inhibition?

Derive the equations for Eddie-Hofstee plot and Lineweaver –Burke plot.

Enlist the energy concerns from heat transfer point of view for a bioreactor.

Write about Non- Newtonian fluids with suitable equations. Explain each of them for its stress and strain relation analogous to the law of viscosity.

Discuss the dynamic method to determine Ka. L

Enlist the enzymes which are medical importance.

Discuss the practical considerations for bioreactor constructions.

Write a note on: Orifice and Nozzle spargers

Discuss aseptic operation by giving schematic to show operating procedures.

Explain the concept of air lift bioreactor. Compare it with fluidized bed bioreactor.

What is the importance of modeling? Where and how does it help in fermentation plants?

Explain the measurement of DO concentrations.

Justify the statement: “Chemostate culture evaluates the kinetic and yield parameters.”

Derive equation to narrate death kinetics of cell culture.

How cells grow? Which factors affect?

Define bioprocess engineering. Explain the role of biotechnology in that.

What are the effects of conditions on Enzyme reaction rate?

Write a note on: Assessing mixing effectiveness

Write the transfer of oxygen from gas bubbles to cell.