Biochemical Engineering-I — Winter 2020

How are cells classified?

What is central dogma of life? Explain in brief.

Derive the equation for batch reaction time required to reduce the substrate concentration from s to s in an enzymatic process. 0 f

What do you mean by recycle stream, bypass stream and purge stream?

Acontinuous process is set up for treatment of wastewater. Each day, 105 kg cellulose and 103 kg bacteria enter in the feed stream, while 104 kg cellulose and 1.5 x 104 kg bacteria leave in the effluent. The rate of cellulose digestion by the bacteria is 7 x 104 kg d-1. The rate of bacterial growth is 2 x 104 kg d-l; the rate of cell death by lysis is 5 x 102 kg d-1. Write balances for Cellulose and bacteria in the system.

Explain different modes of sterilization of liquids.

What do you mean by state properties?

Draw a neat diagram of stirred tank reactor with proper annotations.

Classify the fluids according to their rheological behaviour. Also show their flow curve.

Compare packed bed, fluidized bed and trickel bed reactors.

What is Reynold’s number? Give its equation and significance.

Explain various factors affecting broth viscosity.

Explain the terms bubble formation, gas dispersion and coalescence

Explain Michaelis Menten Kinetics.

Explain role of diffusion in a bioprocess.

What is total reaction rate, volumetric reaction rate and specific reaction rate?

Compare Lineweaver-Burk plot, Eadie-Hofstee plot, Langmuir plot and Direct liner plot.

Explain film theory in detail.

How the Cell Senses Its Extracellular Environment?

What do you mean by overall yield, instantaneous yield, theoretical and observed yield?

Draw and explain Typical batch growth curve and state values of μ in different phases.1

What is μ and k? Give relation between them. max s

What is k ? What are ways to measure it? La

Explain principle and working of Cone-and-Plate Viscometer.